In the present work an attempt has been made to assess the impact of herbicides (Endothal and Terbacil) on statistical relationship between concentrations of herbicides Vs. opercular beatings at two water temperatures (24.0°C±1.0°C and 30.5±1.0°C) and behavioural response in fresh water air breathing siluroid fish, Heteropneustes fossils (Bloch). The behavioural response of the fish towards toxicants were grossly dependent on concentration and length of exposure of herbicides. The mean opercular movements (beatings) decreased with the increasing concentrations of both the herbicides. After such treatment loss of balance, irratic swimming, secretion of copious amount of mucous and loss of pigmentation etc were clearly marked out. The details have been discussed in this paper.

Azadirachtin, a biopesticide derived from neem (Azadirachta indica) is used to control a wide variety of insect pests of agricultural and medical importance. The tissues of Clarias gariepinus intestine was exposed to a sublethal concentration of 0.247ppm azadirachtin for a duration of 5, 10 and 15 days. The histological investigations was found to be highly toxic and the histological alterations increased with increase in duration. The histopathological alterations in the treated intestine tissues exhibited microviffi necrosis, eroded submucosal layer, binucleated cell, enlarged lumen cell, multinucleated necrotic cell, fusion of viffi and mucosal layer, rupture of villi epithelial cells, vacuolization and necrosis. All these histopathological observations indicated that exposure to sublethal concentrations of azadirachtin caused destructive effect in the intestinal tissues of Clarias gariepinus.

In the present work an attempt has been made to study the effect of an organophosphate pesticide, Metasystox on different haematological parameters in an air breathing siluroid fish, Heteropneustes fossilis (Bloch). Treatment of sublethal concentration (4.50ppm) of Metasystox for 96hrs caused significant decrease in RBC counts, Hb content and PCV as compared to control. The total leucocyte counts (TLC) showed rising trend in lower concentration of Metasystox as compared to control but higher concentrations presented an opposite trend i.e decrease in TLC. However, Lymphocytes and Monocytes showed significant increase in their percentage in Metasystox treated animals. The effects were dose dependent. The reason for such changes have been discussed in this paper.

In this study, genotoxic and cytotoxic effects of Bendiocarb were evaluated in the bone marrow of Calotes versicolor, a reptilian model, using chromosomal aberration assay, micronucleus assay and mitotic index. Adult male garden lizards were acclimatized for one week in the laboratory and then injected intraperitoneally with a daily dose of 5.3 mg/kg body weight (1/3rd LD50) of Bendiocarb. Frequencies of CA in the form of gaps increased significantly (1,3 0.005) after 14th day of treatment. Breaks, deletions and additions were increased significantly (P 0.05) after 21st day. MN frequencies increased significantly (1:3 0.05) after 14th day. No significant change in frequencies of MI was observed till 14th day. However, after 21st day MI frequencies decreased significantly (0.05) in the treated group. This study thus, reveals that bendiocarb induces genotoxicity and cytotoxicity in a time dependent manner in the bone marrow cells of Calotes versicolor. The study also reveals the sensitivity of CA, MN and MI as significant bioassay for genotoxicity and cytotoxicity assessment.

The effect of sublethal concentration of an organochlorine pesticide, Kelthane E.C. (5.1 8ppm) and an organophosphate pesticide, Parathion (10.0ppm) on oxygen consumptioni in a fresh water mud eel, Macrognathus aculeatum (Bloch) were studied at various periods of exposures. The rate of the oxygen consumption (ml/hr) increased significantly during the first 36-hrs exposure which later on decreased gradually in Kelthane exposure. Parathion significantly decreased the oxygen consumption during different period of exposure. The reason for such changes have been discussed in this paper.

The medicinal plants contain various chemical compounds which affect and alter the membrane of human Erythrocytes. In the present study, the comparative in-vitro haemolytic activity of various extracts of Aloevera has been evaluated. The Methanolic (AVM), Ethylacetate (AVE), Chloroform (AVC) & Benzene (AVB) was assayed on human Erythrocytes with different concentration. The various extracts of Aloeverawas tested on human Erythrocytes for haemolytic activity with expose of increasing concentration of Aloevera. The expose concentration was 0- 600pg. The result show that Aloevera has no haemolytic activity at these concentrations.

In the present study, anti-leishmanial activity of aqueous extract of Tinospora cordifolia has been evaluated. The aqueous extract of Tinospora cordifolia inhibit the growth of promastigote form of Leishmania donovani . Berber in present in Tinospora cordifolia may be responsible for inhibition of growth of promastigote of Leishmania donovani. The aqueous extract of Tinospora cordifolia showed anti leishmanial activity and it was found that LC„ of 300 pg/ml i-e 50% inhibition at 300 pg/ml& LC. of 400 pg/ml i-e. complete inhibition at400 pg/ml.

In the present work an attempt has been made to study the effect of an organophosphate pesticide, Metacid-50 on different blood parameters in an air breathing siluroid fish Mystus vittatus (Bloch). Treatment of acute sublethal concentration of Metacid (96hrs exposure) caused significant decrease in RBC counts, Hb content and PCV values as compared to control. The TLC showed rising trend in lower concentration of Metacid as compared to control but in higher concentration it (TLC) showed an opposite trend i.e. decline in TLC. However, Lymphocytes and Monocytes showed significant increase in their percentage. The effects were dose dependent. The reason for such changes have been discussed in detail.

The present work was carried out during summer and rainy period on Ranchi Lake. Qualitative analysis Zooplankton population of the lake indicates occurrence of 20 species during both summer and rainy seasons. The major groups of Zooplankton in descending order are Copepoda, Cladocera, Rotifera, and Ostracoda. Higher density of Zooplankton population was noted during rainy season. Among the 19 Zooplanktonic species, 4 form Protozoa, 5 from Rotifera, 4 from Cladocera and 3 each from Ostracoda and Copepoda have been recorded. Simultaneously physico-chemical parameters such as both atmospheric and surface temperature, transparency, pH, CO2, and DO values were recorded. All the values of physico­chemical parameters showed slight variations during the summer and rainy seasons.

The present study was conducted on DNA extraction and determination of DNA quantity of both male and female of Catla catla using Nanophotometer. The fishes were divided into three different groups (group I, group II, group Ill) and fishes of group I were collected from the water bodies of Bijnor district, group 11 from district Hapur and group Ill from KilaParikshit Garh (District Meerut), of Western Uttar Pradesh of India. DNA was isolated by using the method of Ruzzante et. al., (1996) with minor modifications. Gel electrophoresis was carried out and extracted DNA was analyzed using nanospectrophotometer (Nanophotometer P330; Implen, Germany) to determine the quantity of DNA and its purity level. DNA extraction was done and extracted DNA was analyzed using nanophotometer to determine the concentration of DNA and its purity level. The value of DNA concentration in group r female of Catla catla was between 62 and 68 ng/pl and of male was between range of 71- 78 ng/pl. The value of DNA concentration in group if d female of Catla catla was 62 and 65 ng/pl and of male 77- 80 ng/pl. Highest DNA concentration was seen in group lir, in females the concentration was between 78-84 ng/pl and in case of males value was between 88-94 ng/pl.