The changes of reproductive cycle in higher vertebrate mammalian rodent give a reasonable index of its ovarian activity with hormonal synthesis of estrogens and progesterone. In the present work an attempt has been made to study the assessment of different concentration of silk dye effluent on reproductive physiology of female Albino mice, Mus musculus. The reproductive toxicity was observed in 25%, 50%, 75% and 100% concentrations. The silk dye effluent cause significant effect on estrous cycle, copora lutea, implantation site, gestation length, litter count, percent survival of litter count and percent pre Implamentational loss. The control mice exhibited regular estrous cycle of 4-5 days. All the parameters except estrus cycle and gestation length reduced with increase in effluent concentration. When female mice treated with different concentration cause a significant decrease in the number of estrus cycle. The numbers of corpora lutea were significantly reduced from 14-5, implementation site 11-2, litter count 11-1.2 and percent survival of litter count from 95.605% to 32.166 % while the percent pre implantational loss were increased from 10 to 36.744 and gestation lengths increased with the increase in effluent concentration from 19.8 – 21.1 days. The finding indicated that the reproductive fertility and ability severely affected due to the different concentrations of silk dye effluent. The detail report of the investigation is presented in this paper.