Blood pressure is one of the most important risk factor for attributable burden of disease. But the percentage of treated patients had their BP under control is not satisfactory. Utilization of ACE inhibitors is one of the important strategies for maintenance of BP. T cordifolia leaf extract was analyzed to find a new ACE regulatory compound in in-vitro. Among the extracted compounds, 8,11,14-eicosatrienoic acid showed highest affinity towards the enzyme and can be used as a potent ACE regulatory compound to manage BP The results of this experiment clearly indicate that the plant extract activates the ACE activity in in-vitro condition.