Catfishes globally find importance due to high demand as human food, angling sport and aquariums purpose. In India, catfishes are found in abundance and are equally exploited through decades. In this study, five families viz. Sisoridae, Siluridae, Clariidae, Bagridae and Heteropneustidae representing 6 genera, 16 species were subjected to phylogenetic analyses of sequence data for the mitochondrial gene cytochrome b. The pairwise genetic distance among catfishes based on cytochrome b was found maximum between Mystus bleekeri and Clarias magur (28.5%), minimum between Glyptothorax ventrolineatus and Glyptothorax granules (5.2 %) and overall mean genetic distance among cat fishes for cytochrome b was 0.208 via composite maximum likelihood method. The overall Conserved site, Variable site, Parsim-info site and Singleton site of Cytochrome b gene in cat fish species were found 265/479, 214/479, 168/479 and 46/479. The genus of family Siluridae, Clariidae, Sisoridae respectively formed separate monophyletic clade in phylogenetic tree. Clariidae and Heteropneustidae species found in a same clade which shows that it has some evolutionary relationship. The evolutionary history of family Sisoridae, Siluridae, Clariidae, and Heteropneustidae will provide the foundation to understand the origin and diversification of most of the species of catfish in India.