The medicinal plants contain so many chemical compounds which has toxic effect on human Erythrocytes(RBCs).In the present study, the comparative In vitro haemolytic activity of different extracts of Swertia chirata has been evaluated. The Methanolic (SCM), Ethylacetate (SCE), chloroform (SCC) & Benzene (SCB)extract was assayed on human Erythrocytes with different concentration. The different extracts of Swertia chirata was tested on human erythrocytes for hemolytic activity with exposure of increasing concentration . The exposed concentration was 0- 300 µg. The result shows that the different extracts of Swertia chirata has no effect on human erythrocytes (RBCs)at 0- 300 µg.