The species of family Drosophilidae is a well known and documented experimental organism in almost in every field of biological science. Revolving around the study of geographical distribution and species diversity of this family, seven districts of North Chotanagpur were surveyed which revealed the occurrence of a total 2909 flies. Taxonomically, the collected species belong to four different genera and twenty species. Moreover, the species are more abundant and diversified in Hazaribag showing negative correlation with the temperature (r = -0.824) and positive correlation with the altitude (r = 0.94). The least abundance was shown in Dhanbad. The result of biodiversity indices indicates the species diversity in the order of HZ> KO> GR> BK> DN> CH> RM.