Infections due to protozoa of the genus Leishmania are a major worldwide health problem, with high endemicity in developing countries: Visceral Leishmaniasis commonly called Kala-azar which is caused by an intracellular parasite Leishmania donovani. This disease may be fatal when left untreated. In the present study, in vitro anti- leishmanial activity of Ethyl acetate, Methanolic, Benzene and Chloroform extracts of Swertia chirata have been evaluated. Among those extracts Methanolic extracts of Swertia chirata shows effective antileishmanial activity against promastigote form of Leishmania donovani. Amarogentin present in Swertia chirata which may be responsible for inhibiting the growth of parasite. It was found that LC =150 50 µg/ml i.e. 50% inhibition at 100 µg/ml and LC =200µg/ml for complete inhibition is required to inhibit the 90 growth of Leishmania donovani..