The present paper deals with the effect of hormones and pharmacological agents on the activity of gonads in Anabas testudineus at two different temperatures and different periods of breeding cycles. In males at high temperature 31°C, in May when the testes were matured and full of sperms, the treatment of ovocyclin, duogynon forte and testosterone propionate (T.P.) brings about significant decrease in 68! as compared to control indicating that these agents play an important role in the release of milt but at low temperature (22°C, in January) when the testes were in regressed condition except TP. all the agents failed to exhibit any significant change in the testicular activity. Fertyl in males and ovocyclin in females has no effect on the gonadal activity. At high temperature (31°C) the treatment of lutocyclin, fertyl and duogynon forte brings about significant decrease in G8! in females indicating that these agents are concerned with the maturation and finally ovulation of eggs but at low temperature except fertyl all the the agents failed to bring any significant change in GSI. The effect of different agents seems to be temperature dependent.