Use of plants in synthesis of nanoparticles is quite novel approach leading to ‘green chemistry’. This green chemistry has many advantages such as, process scaling up, economic viability and safe way to produce nanoparticles. Now the study focused on the production, characterization, physical parameters and antimicrobial studies of silver nanoparticles using Ficus elastic rubra leaf extract under ambient conditions. In the present study, a broad peak appeared at 430nm, which indicates the uniform distribution and poly dispersion of silver nanoparticles (AgNPs) rose from the extract of Leaf concoction of Ficus elastic rubra. It serves as a potent antimicrobial agent against E.coli, Corynebacterium, S.aureus and P.aeruginosa exhibiting significant Pharmacological activity when compared to standard Drug Ciprofloxacin. In futureFicus elastic rubra nanoparticles may gain wide applications in medicine.