The medicinal plants synthesise numerous phytochemicals which have potential biological activity, which affect and alter the membrane of human Erythrocytes. In the present study, the comparative an invitro haemolytic study of different extracts of Agave americana has been evaluated. The Methanolic, Ethyl acetate, Chloroform and Benzene was assayed on human erythrocytes(RBCs) with different concentration. The various extracts of Agave americana on human erythrocytes(RBCs) for haemolytic activity with expose of increasing concentration The exposed concentration was 0-500fjg. Ethyl acetate extracts of Agave americana showed haemolytic activity and it was found that IC50 at 300 ^ig and IC90 at 350fjg.